phoP, SPI1, SPI2 and aroA mutants of Salmonella Enteritidis induce a different immune response in chickens

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phoP, SPI1, SPI2 and aroA mutants of Salmonella Enteritidis induce a different immune response in chickens

Poultry is the most frequent reservoir of non-typhoid Salmonella enterica for humans. Understanding the interactions between chickens and S. enterica is therefore important for vaccine design and subsequent decrease in the incidence of human salmonellosis. In this study we therefore characterized the interactions between chickens and phoP, aroA, SPI1 and SPI2 mutants of S. Enteritidis. First we...

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Vaccination of Chickens with SPI1-lon and SPI1-lon-fliC Mutant of Salmonella enterica Serovar Enteritidis

The prevalence of Salmonella enterica serovar Enteritidis is gradually decreasing in poultry flocks in the EU, which may result in the demand for a vaccine that allows for the differentiation of vaccinated flocks from those infected by wild-type S. Enteritidis. In this study, we therefore constructed a (Salmonella Pathogenicity Island 1) SPI1-lon mutant with or without fliC encoding for S. Ente...

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Invasiveness and persistence of Salmonella enteritidis, Salmonella typhimurium, and a genetically defined S. enteritidis aroA strain in young chickens.

Newly hatched chicks were dosed orally with a Salmonella typhimurium wild-type strain, an S. enteritidis wild-type strain, and a genetically defined S. enteritidis aroA vaccine candidate, strain CVL30. The S. typhimurium strain, 2391 Nalr, was virulent in newly hatched chicks and caused deaths in 7 of 20 chicks after an oral dose of 10(5) organisms. The S. enteritidis wild-type strain, LA5, cau...

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Extracellular antigens from Salmonella enteritidis induce effective immune response in mice after oral vaccination.

We have studied polyacryl starch microparticles as an adjuvant in oral vaccination in mice. Secreted antigens from Salmonella enterica serovar Enteritidis were administered covalently conjugated to microparticles, or as free antigens, orally or intramuscularly and evaluated for their immunogenicity and ability to elicit protective immune response against an oral challenge with live serovar Ente...

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Dynamics of Lymphocyte Subpopulations in Immune Organs of Chickens Infected with Salmonella enteritidis

Asheg A. A. , M. Levkut , V. Revajová, Z. ·evãíková, L. Kolodzieyski , J . Pis t l : Dynamics of Lymphocyte Subpopulations in Immune Organs of Chickens Infected with Salmonella enteritidis. Acta Vet. Brno 2003, 72: 359-364. To determine the effect of different doses of Salmonella enteritidis on immunocompetent cells, the thymus, bursa of Fabricius, and peripheral blood were examined. One-day-ol...

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ژورنال

عنوان ژورنال: Veterinary Research

سال: 2015

ISSN: 1297-9716

DOI: 10.1186/s13567-015-0224-x